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15195831611
服务热线
15195831611
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| 产地 | |
| 保存条件 | 2-8℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | 09-038-I |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μg |
| 是否进口 | 是 |
| Description | |
|---|---|
| Catalogue Number | 09-038-I |
| Replaces | 09-038 |
| Description | Anti-SOX17 |
| Alternate Names |
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| Background Information |
Transcription factor SOX-17 (UniProt: Q61473; also known as SOX-17) is encoded by the Sox-17 gene (Gene ID: 20671) in murine species. Sox17 protein is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. It binds to the sequences 5'-AACAAT-3 or 5'-AACAAAG-3' and modulates transcriptional regulation via WNT3A. Sox17 is reported to act upstream of the Notch system and downstream of the canonical Wnt system and acts as a link between the canonical Wnt and Notch signaling systems. It is involved in the regulation of the formation of cells that go on to develop into endodermal tissues like pancreatic and liver cells. SOX17 is the key regulator of human primordial germ cells (hPGSc) fate and is considered as one of the earliest markers. It is also shown to be the transcription factor for the regulation of oligodendrocyte progenitor cells, maintenance of fetal hematopoeitic stem cells, and in the mediation of cardiac muscle cell formation. SOX17 is selectively expressed in arterial endothelial cells at the early stages of embryonic development and maintains the same distribution postnatally and in the adult. Inactivation of SOX17 in endothelial cells in mouse embryo leads to a lack of arterial differentiation and vascular remodeling, which that results in embryo death in utero. (Ref.: Corada, M., et al. (2013). Nat. Communications 4, 2609; Niakan, KK et al. (2010). Gene Dev. 24(3): 312-326). |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation |
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-SOX17, Cat. No. 09-038-I, is a highly specific rabbit polyclonal antibody that targets Transcription factor SOX-17 and has been tested in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting. |
| Key Applications |
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| Application Notes |
Western Blotting Analysis: 2 μg/mL from a representative lot detected SOX17 in 10 μg of human spinal cord tissue lysate. Immunofluorescence Analysis: A 1:150-300 dilution from a representative lot detected SOX17 in human testis and rat colon tissues. Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected SOX17 in human prostate, mouse stomach, and rat uterus tissues. |
| Biological Information | |
|---|---|
| Immunogen |
KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of murine Sox17. |
| Concentration | Please refer to lot specific datasheet. |
| Host | Rabbit |
| Specificity |
This polyclonal antibody detects Sox17 in human, mouse, and rat. It targets an epitope within 17 amino acids from the N-terminal region. |
| Species Reactivity |
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| Species Reactivity Note |
Human, Mouse, Rat |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Affinity Purified |
| UniProt Number |
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| Molecular Weight | ~40 kDa observed; 44.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance |
Evaluated by Western Blotting in H9 human stem cells. Western Blotting Analysis: 2 μg/mL of this antibody detected SOX17 in 10 μg of H9 human stem cells lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions |
Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |
