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15195831611
服务热线
15195831611
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| 产地 | |
| 保存条件 | 2-8℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | MAB4419 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μg |
| 是否进口 | 是 |
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, FC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4419 |
| Replaces | MAB4305 |
| Description | Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 |
| Alternate Names |
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| Background Information | Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified mouse monoclonal IgG1κ in a buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% NaN3. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 is validated for use in IC, FC, WB for the detection of OCT-4 [POU5F1]. |
| Key Applications |
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| Application Notes |
Western Blotting: Recommended working dilution is 1 - 2 μg/mL. Flow Cytometry: Recommended working dilution is 2 μg/mL. Immunocytochemistry: Recommended working dilution is 1:100 – 1: 200. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human Oct-4 protein, a.a. 1-145. |
| Clone | 7F9.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with the intracellular protein, Oct-4 and detects the Oct-4A isoform. |
| Isotype | IgG1κ |
| Species Reactivity |
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| Species Reactivity Note | Reacts with both human and mouse Oct-4; however, reactivity to other species has not been determined. |
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G sepharose column |
| UniProt Number |
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| UniProt Summary |
FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. SUBUNIT STRUCTURE:Interacts with UBE2I. SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern. TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues. DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1 INDUCTION: Repressed by retinoic acid (RA). PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. |
| Stem Cell Type |
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| Molecular Weight | ~39 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |
