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Q5® High-Fidelity 2X Master Mix
  • 品牌:New England Biolabs
  • 型号:100 reactions (50 μl)
  • 货号:M0492S
  • 价格: ¥1699/盒
  • 发布日期: 2023-07-06
  • 更新日期: 2024-08-30
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产地
品牌 New England Biolabs
货号 M0492S
用途
英文名称
包装规格 100 reactions (50 μl)
纯度 %
CAS编号
别名
分子式
是否进口

The Q5 High-Fidelity 2X Master Mix offers robust, high-fidelity performance in a convenient master mix format. The Q5 High- Fidelity 2X Master Mix features a high-fidelity, thermostable DNA polymerase with 3′→ 5′ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 High-Fidelity Master Mix contains 2 mM Mg++. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator  is highly recommended.

Q5-High Fidelity 2X Master Mix formats allow robust amplification of a broad range of targets with a single formulation. 
M0492Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity 2X Master Mix. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.

产品来源

An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.产品类别: Q5® High-Fidelity DNA Polymerases Products,  Master Mixes Products 应用: Long Range PCR,  Fast PCR,  High-Fidelity PCR,  | More +