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Q5® Hot Start High-Fidelity DNA Polymerase
  • 品牌:New England Biolabs
  • 型号:100 units
  • 货号:M0493S
  • 价格: ¥1279/盒
  • 发布日期: 2023-07-06
  • 更新日期: 2024-08-30
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产地
品牌 New England Biolabs
货号 M0493S
用途
英文名称
包装规格 100 units
纯度 %
CAS编号
别名
分子式
是否进口

Q5® Hot Start High-Fidelity DNA Polymerase is a high-fidelity, thermostable, hot start DNA polymerase with 3′→ 5′ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 Hot Start High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 Hot Start High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator  is highly recommended.

M0493Amplification of a variety of human genomic amplicons from low to high GC content using Q5 Hot Start High-Fidelity DNA Polymerase. All reactions were set up at room temperature, conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.

 

产品来源

An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.产品类别: Q5® High-Fidelity DNA Polymerases Products 应用: Genome Editing Applications,  Hot Start PCR,  Long Range PCR,  | More +