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产品名称:
Peanut Agglutinin (PNA), Biotinylated, 5 mg/花生凝集素，生物素标记
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别名:
花生凝集素，生物素标记
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产品描述:
Peanut agglutinin PNA binds preferentially to the T-antigen, a galactosyl (β-1,3) N-acetylgalactosamine structure present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. With certain exceptions, the receptor sequence for PNA is normally sialylated which prevents the lectin from binding to its receptor oligosaccharide (see Jacalin). Even sialic acid which is not bound directly to the receptor sugars may inhibit binding. The presence of calcium ions in diluents can enhance the binding of PNA to receptors, possibly by neutralizing the negative charges on sialic acid residues adjacent to the receptor sequence.Biotinylated peanut agglutinin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium sodium azide.
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实验应用:
Immunohistochemistry / Immunocytochemistry,Immunofluorescence,Blotting Applications,Elispot,ELISAs,Glycobiology
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推荐稀释度:
DO NOT VORTEX. For most applications we recommend a freshly prepared working solution of 5-20μg/ml in the above buffer. Use of buffers containing 0.1 mMCaCl2 and 0.01 mM MnCl2 is recommended. Inhibiting/Eluting Sugar: 200 mM galactose (S-9003).
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偶联:
Biotinylated
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产品浓度:
5 mg active conjugate/ml
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存储溶液:
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2
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产地:
美国
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样本类型:
10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2, 0.01 mM MnCl2
服务热线
15195831611
