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HighYield T7 ARCA mRNA Synthesis Kit
  • 型号:50T x 20 μl
  • 货号:RNT-102-L
  • 价格: ¥8175/盒
  • 发布日期: 2023-02-16
  • 更新日期: 2024-08-30
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产地
品牌
货号 RNT-102-L
用途
英文名称
包装规格 50T x 20 μl
纯度 %
CAS编号
别名
分子式
是否进口

产品信息

品牌

货号

名称

规格

单价

Jena Bioscience

RNT-102-L

HighYield T7 ARCA mRNA Synthesis Kit

50T x 20 μl

8175.00

产品简介

      HighYield T7 ARCA mRNA合成试剂盒旨在通过与T7 RNA聚合酶的体外转录产生大量ARCA帽(m)RNA。由此产生的5’-帽(m)RNA随后可用于微注射、转染或体外翻译实验。与传统的m7GpppG-caped(m)RNA相比,抗逆转录帽类似物(ARCA,m7,3'-OGpppG)caped(m)RNA具有更高的翻译效率。这是由于m7鸟嘌呤部分的3'-O-甲基化,允许ARCA以正确(“反逆转”)方向并入,只产生100%可翻译的带帽(m)RNA群体。

      该试剂盒含有足够的试剂,可用于15个反应(S-Pack)或50个反应(L-Pack)?20μL(6 mM ARCA、1.5 mM GTP、7.5 mM CTP、7.5 mL UTP、7.5 mM ATP)。使用单核苷酸格式,可以轻松实现ARCA浓度的单独优化或修饰核苷酸的合并(例如伪UTP或N4乙酰CTP)高达100%替换。培养30分钟后,20μl反应产生约30-50μg RNA(1μg T7对照模板,1.4 kb RNA转录物)。然而,产量可能因模板而异(启动子设计、序列长度、二级结构形成)。

Content:
HighYield T7 RNA Polymerase Mix
incl. RNase inhibitor and 50 % glycerol (v/v)
#RNT-102-S: 2x 40 μl
#RNT-102-L: 3x 40 μl

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

ARCA - Solution
#RNT-102-S: 2x 10 μl (100 mM)
#RNT-102-L: 6x 10 μl (100 mM)

T7 control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in ~1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I

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