AB3209 .Sigma-Aldrich. Anti-Oct-4 Antibody-南京沃博生物科技有限公司

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AB3209 .Sigma-Aldrich. Anti-Oct-4 Antibody

品牌：Sigma-Aldrich

型号：100 μg

货号：AB3209

发布日期： 2023-05-05
更新日期： 2024-08-30

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品牌	Sigma-Aldrich
货号	AB3209
用途
组织来源
细胞形态
是否是肿瘤细胞
保质期
器官来源
免疫类型
品系
生长状态
物种来源
包装规格	100 μg
是否进口	是

Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	ICC, IHC, WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB3209
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Oct-4 Antibody
Alternate Names	POU5F1
Background Information	Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

Product Information
Format	Affinity Purified
Control	Pluripotent human embryonic stem (ES) cells
Presentation	Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality Level	MQ100

Applications
Application	This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.
Key Applications	Immunocytochemistry Immunohistochemistry Western Blotting
Application Notes	Western blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet. Immunohistochemistry: 1:200-1:1,000. Immunocytochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide, amino acids 282-297of Oct4.
Host	Rabbit
Specificity	Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_002701.4 NM_203289.3
Gene Symbol	POU5F1 Oct-3 OTF4 OCT3 OCT4 Oct-4 MGC22487 Oct4 OTF3
Purification Method	ImmunoAffinity Purified
UniProt Number	Q01860
UniProt Summary	FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene. SIZE: 360 amino acids; 38571 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels. SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain. MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
Stem Cell Type	Human Embryonic Stem Cells Mouse Embryonic Stem Cells Induced Pluripotent Stem Cells
Molecular Weight	44 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 μg