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15195831611
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15195831611
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| 产地 | |
| 保存条件 | -20℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | ESG2207 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 10? units |
| 是否进口 | 是 |
| Key Applications | Source | Purity |
|---|---|---|
| Cult | rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography. | The active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE. |
| Description | |
|---|---|
| Catalogue Number | ESG2207 |
| Brand Family |
Chemicon® |
| Trade Name |
|
| Description | ESGRO® Recombinant Rat LIF Protein |
| Overview | Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF. |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation |
Purified, sterile liquid. Rat ESGRO® is supplied in liquid form as 107 Units ESGRO® in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability. Rat ESGRO® is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | ESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^7 units/ml |
| Key Applications |
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| Application Notes |
Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et al. 1998). Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et al. 1998). At this recommended concentration 106 units of ESGRO® is sufficient for 1L of tissue culture media. |
| Biological Information | |
|---|---|
| Concentration | 10 million units/1 mL |
| Purity | The active component rat LIF (rtLIF) has been shown to be >85% pure by SDS-PAGE. |
| Source | rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography. |
| Specific Activity |
Rat ESGRO® is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells. A standard of 50 Units is defined as the concentration of Rat ESGRO® in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies. 106 units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity > 108 units/mg. Note: LIF should not be allowed to come in contact with ruminant or swine. |
| Entrez Gene Summary | Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary |
FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 202 amino acids; 22008 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family. |
| Media Form | Liquid |
| Stem Cell Type |
|
| Product Usage Statements | |
|---|---|
| Quality Assurance | The highest standards of quality control are used in the manufacture of this product. No warranty is provided that the sale or use of the product either alone, in combination with other products, or in the operation of any process, will not infringe patent, intellectual property or any other rights of third parties. This product is supplied for research purposes only. It is not for human therapeutic and/or diagnostic use. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions |
Rat ESGRO® is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that Rat ESGRO® concentrate be stored at -20°C. It is recommended that prior to use, Rat ESGRO® should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at -20°C. Freeze thawing will reduce potency and is not recommended. |
| Packaging Information | |
|---|---|
| Material Size | 10? units |
| Material Package | 1 mL |
