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15195831611
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15195831611
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| 产地 | |
| 保存条件 | -20℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | MAB1281 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μL |
| 是否进口 | 是 |
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Human Only | IH(P), IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1281 |
| Brand Family |
Chemicon® |
| Trade Name |
|
| Description | Anti-Nuclei Antibody, clone 235-1 |
| Alternate Names |
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| Background Information | Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Hybridoma supernatant. Liquid in PBS. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP. |
| Key Applications |
|
| Application Notes |
Immunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500. Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended. Identifies an 82 and 70 kD protein by immunoprecipitation. Optimal working dilutions and protocols must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | 235-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Species Reactivity Note | The human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity. |
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein A Purfied |
| Stem Cell Type |
|
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Packaging Information | |
|---|---|
| Material Size | 100 μL |
