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15195831611
服务热线
15195831611
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| 产地 | |
| 保存条件 | |
| 品牌 | Sigma-Aldrich |
| 货号 | MAB1281A4 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μL |
| 是否进口 | 是 |
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ICC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1281A4 |
| Description | Anti-Nuclei Antibody, clone 235-1, Alexa Fluor® 488 conjugate |
| Alternate Names |
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| Background Information | Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat. |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | The anti-Nuclei, clone 235-1, Alexa Fluor 488 conjugate will recognize human cell nuclei only & is usefull in xenograph transplantation studies to study stem cell migration. |
| Key Applications |
|
| Application Notes | Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in H9 human embryonic stem cells. Evaluated by Immunocytochemistry in mouse embryonic fibroblasts. Immunocytochemistry Analysis: A 1:50 dilution of this antibody did not detect nuclei in mouse embryonic fibroblasts. |
| Biological Information | |
|---|---|
| Immunogen | Human nuclei |
| Clone | Clone 235-1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Purification Method | Protein G Purified |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in human foreskin fibroblasts (SCC058). Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected nuclei in human foreskin fibroblasts (SCC058). |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μL |
