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Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA
  • 品牌:Sigma-Aldrich
  • 型号:100 μg
  • 货号:MAB4385
  • 发布日期: 2023-05-05
  • 更新日期: 2024-08-30
产品详请
产地
保存条件 2-8℃
品牌 Sigma-Aldrich
货号 MAB4385
用途
组织来源
细胞形态
是否是肿瘤细胞
保质期
器官来源
免疫类型
品系
生长状态
物种来源
包装规格 100 μg
是否进口

Key Spec Table

Species Reactivity Key Applications Host Format Antibody Type
H FC, IP, WB M Purified Monoclonal Antibody
Description
Catalogue Number MAB4385
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85
Background Information The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.
Product Information
Format Purified
Control
  • Hemopoietic cells and a wide range of normal human tissues
Presentation Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide
Quality Level MQ100
Applications
Application Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blotting
Application Notes FACS Analysis: a starting range of 5-20 μg/ml is suggested.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen 2102Ep human Embryonal Carcinoma Cells
Epitope blood group antigen Ok(a)
Clone TRA-1-85
Host Mouse
Specificity This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.
SPECIES REACTIVITIES:
Refer to comments regarding specificity
Isotype IgG1
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Gene Symbol
  • TRA-1-85
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight 35-69 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months
Packaging Information
Material Size 100 μg