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15195831611
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| 产地 | |
| 保存条件 | 2-8℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | MAB4419A4 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μL |
| 是否进口 | 是 |
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC | M | AlexaFluor®488 | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4419A4 |
| Description | Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor® 488 conjugate |
| Alternate Names |
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| Background Information | Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 has been identified as one of the main transcription factors required to reprogram somatic cells into induced pluripotent stem cells (iPS cells). |
| Product Information | |
|---|---|
| Format | AlexaFluor®488 |
| Control |
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| Presentation | Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15mg/ml BSA. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor 488 conjugate is an antibody against OCT-4 [POU5F1] for use in ICC. |
| Key Applications |
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| Application Notes | Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Oct-4 in H9 human embryonic stem cells. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant human Oct-4 protein, aa 1-145 |
| Clone | clone 7F9.2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes human and mouse Oct-4. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | The uncojugated parent antibody (Catalog No. MAB4419) has an observed MW at 39 kDA. |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Oct-4 in mouse embryonic stem cells (SCR012). |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μL |
