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15195831611
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15195831611
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| 产地 | |
| 保存条件 | 2-8℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | MABD116 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μL |
| 是否进口 | 是 |
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, EMSA, IP | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABD116 |
| Description | Anti-Dux4 Antibody, clone 9A12 |
| Alternate Names |
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| Background Information | Dux4 is a homeodomain protein with a similar protein sequence to Pax3 and Pax7. Defects in Dux4 may be the cause of facioscapulohumeral muscular dystrophy (FSHD). FSHD is characterized by weakness of the muscles of the face, upper-arm and shoulder girdle. Ectopic expression of Dux4 in skeletal muscle activates the expression of stem cell and germline genes, and, when overexpressed in somatic cells, Dux4 can ultimately lead to cell death. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Dux4 using this mouse monoclonal antibody, Anti-Dux4 Antibody, clone 9A12 validated for use in western blotting, ICC, Electrophoretic Mobility Shift Assay & IP. |
| Key Applications |
|
| Application Notes |
This antibody has been shown to work in immunocytochemistry applications (Dixit et al. 2007). This antibody has been shown to work in electrophoretic mobility shift assays (Dixit et al. 2007). This antibody has been shown to work in immunoprecipitation applications (Anseau et al., 2009). |
| Biological Information | |
|---|---|
| Immunogen | recombinant protein corresponding to the domain of: C-Terminal region of Human Dux4. |
| Epitope | domain of: C-Terminal region |
| Clone | 9A12 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Isotype | IgG1κ |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | Protein G Purified |
| UniProt Number |
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| Molecular Weight | ~58 kDa observed |
| Product Usage Statements | |
|---|---|
| Quality Assurance |
Evaluated by Western Blot in a human skeletal muscle tissue lysate. Western Blot Analysis: A 1:250 dilution of this antibody detected Dux4 in a human skeletal muscle tissue lysate. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μL |
