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15195831611
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15195831611
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| 产地 | |
| 保存条件 | -20℃ |
| 品牌 | Sigma-Aldrich |
| 货号 | MABD167 |
| 用途 | |
| 组织来源 | |
| 细胞形态 | |
| 是否是肿瘤细胞 | |
| 保质期 | |
| 器官来源 | |
| 免疫类型 | |
| 品系 | |
| 生长状态 | |
| 物种来源 | |
| 包装规格 | 100 μL |
| 是否进口 | 是 |
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC, IHC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABD167 |
| Replaces | MABE470 |
| Description | Anti-GATA1 Antibody, clone 4F5 |
| Alternate Names |
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| Background Information |
GATA-1 (GATA1), or alternatively Gata-binding factor 1, GF-1, NF-E1 DNA binding protein, and Erythroid transcription factor (EryF1) encoded by the human gene named GATA1/ERYF1/GF1 is transcriptional activator central for erythroid development. GATA1 also plays critical roles in controlling gene expression in other system as well. Research has shown that GATA1 contributes to the level of alpha synuclein gene expression in Parkinson’s disease and over expression of GATA1 in cortical neurons leads to a decrease in the expression of Rab4b and reduces dendrite branching and maturation, and GATA1 expression is elevated in brains with depressive disorder suggesting that GATA1 may be new target for anti-depressant therapies. EMD-Millipore’s Anti-GATA1 mouse monoclonal antibody has been tested in western blot on K562 cell lysates and paraffin embedded immunohistochemistry on pancreatic cancer tissues and in fluorescent immunocytochemistry on K562 cells in culture. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
|
| Presentation | Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC. |
| Key Applications |
|
| Application Notes |
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in K562 cells. Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in pancreatic cancer tissue. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant fragment of human GATA1 expressed in E. Coli. |
| Clone | 4F5 |
| Host | Mouse |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~45 kDa observed. Uncharacterized bands may appear in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance |
Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GATA1 in K562 cell lysate. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions |
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μL |
