Bleomycin Sulfate, a glycopeptide antibiotic, is an anticancer agent for squamous cell carcinomas (SCC). In UT-SCC-19A cells, the IC50 of Bleomycin Sulfate is 4 nM.

In a Bleomycin-induced mouse model, BLM was found to promote the development of inflammation, with the increase of TGF-β1, Smad3, and STAT1 leading to severe pulmonary fibrosis.

ADIPO-P2 cells are grown in D-MEM high glucose medium supplemented with 20% fetal calf serum, penicillin (100 U/mL) and streptomycin (100 μg/mL) at 37 °C and 5% CO2 atmosphere. Cells are cultured as monolayer in TC25 Corning flasks containing 1.5 × 105 cells/mL. For each experiment, two flasks are set up, one for the control and one for the treated culture. During the log phase of growth ADIPO-P2 cells are treated with a 30 minutes pulse of 2.5 μg/mL of Bleomycin sulfate. Control cultures are set up in parallel but not exposed to Bleomycin sulfate. Time of exposure and concentration of Bleomycin sulfate are chosen according to previous studies carried out in our laboratory with mammalian cells exposed to Bleomycin sulfate. At the end of the pulse treatment with Bleomycin sulfate, the cells are washed twice with Hank's balanced salt solution and kept in culture with fresh culture medium until harvesting. Cells are continuously maintained in culture during 5 passages or subcultures after treatment. Subcultivation is carried out whenever the cultures became confluent (approximately 4 × 105 cells/mL of culture medium). To estimate cell growth, at the time of subcultivation cells are collected by trypsinization, an aliquot of about 200 μL stained with 0.4% trypan blue, and the number of viable cells is determined. Cells are then suspended in fresh culture medium and dispensed into new culture flasks containing 1 × 105 cells/mL to continue growing. The rest of the cells is discarded or dispensed in another flask for cytogenetic analysis, which is performed at 18 hours and 10 days after the end of treatments. To analyze chromosomal aberrations, colchicine (0.1 μg/mL) is added to cell cultures during the last 3 hours of culture. Chromosome preparations are made following standard procedures. After harvesting, cells are hypotonically shocked, fixed in methanol:acetic acid (3:1), spread onto glass slides and processed for PNA-FISH.Two independent experiments are carried out. (Only for Reference)

9041-93-4

C55H85N17O25S4

1512.62

硫酸博来霉素;NSC125066;硫酸博莱霉素;Blenoxane;Bleomycin Sulfate

[1]Liang J, Niu Z, Zhang B, et al. Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis[J]. Cell Death & Differentiation. 2020: 1-15. [2]J??skel?-Saari HA, et al. Acta Otolaryngol Suppl. 1997;529:241-4. [3]Liang J, Niu Z, Yu X, et al. Counteracting Genome Instability by p53-dependent Mintosis[J]. bioRxiv. 2020. [4]Corboz MR, et al. Therapeutic administration of inhaled INS1009, a treprostinil prodrug formulation, inhibits bleomycin-induced pulmonary fibrosis in rats. Pulm Pharmacol Ther. 2018 Apr;49:95-103. [5]Zongwang Zhang1,2 , Yanwei Wu2 , Bing Wu2,3, etal, Lili Niu1* and Wei Tang2,3. DZ2002 ameliorates fibrosis, inflammation,and vasculopathy in experimental systemic sclerosis models. Arthritis Research & Therapy. (2019) 21:290 [6]Cort A, et al. Mol Med Report. 2012, 5(6), 1481-1486. [7]Hovhannisyan G, et al. Comparative analysis of individual chromosome involvement in micronuclei induced by bleomycin in human leukocytes. Mol Cytogenet. 2016 Jun 21;9:49. [8]Denholm EM, et al. Am J Pathol. 1989, 134(2), 355-363. [9]Paviolo NS, et al. Mutat Res. 2012, 734(1-2), 5-11. [10]Banerjee ER, et al. Stem Cell Res Ther. 2012, 3(3), 21.

[1]Yi X M, Li M, Chen Y D, et al. Reciprocal regulation of IL-33 receptor–mediated inflammatory response and pulmonary fibrosis by TRAF6 and USP38. Proceedings of the National Academy of Sciences. 2022, 119(10): e2116279119 [2]Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis. Cell Death & Differentiation. 2020: 1-15 [3]Liang J, Niu Z, Zhang B, et al Liang J, Niu Z, Zhang B, et al. p53-dependent elimination of aneuploid mitotic offspring by entosis. Cell Death & Differentiation. 2020: 1-15.

Ethanol:<1 mg/mL
DMSO:93 mg/mL (61.5 mM)
H2O:92 mg/mL (60.8 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years